OptiPrep, 250 grams
Simplify the isolation and purification of macromolecules; viruses; a wide range of cell types; and organelles such as nuclei, mitochondria, endosomes, or exosomes by using OptiPrep™ medium. This flexible, versatile, and gentle density gradient medium is non-ionic, iodixanol-based (60% w/v) and has a density of 1.320 ± 0.001 g/mL. OptiPrep™ has been used clinically in X-ray contrast imaging and has been shown to have lower toxicity when compared to ionic gradient compounds such as metrizamide.
• Iodixanol: 60% (w/v)
• Density: 1.320 ± 0.001 g/mL
Mononuclear Cells, Other
Human, Mouse, Rat, Non-Human Primate, Other
AREA OF INTEREST
OptiPrep™ is a sterile and endotoxin tested solution of 60%
iodixanol in water with a density of 1.32 g/ml.
Iodixanol was developed as an X-ray contrast medium an has therefore been subjected to rigorous clinical testing.
Iodixanol is non-ionic, non-toxic to cells and metabolically inert.
Iodixanol solutions can be made isoosmotic at all useful densities.
Iodixanol solutions have low viscosity and osmolality.
Actual endotoxin levels in each batch are usually measured at < 0.13 EU/ml.
The high density of OptiPrep™ facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient or through a simple density barrier. 55 protocols available for cell isolation.
Improved resolution of cell organelles.
Low viscosity, isoosmotic gradients
provide rapid and efficient separation
of the major organelles in preformed