QuantiFluor® dsDNA System
The QuantiFluor® dsDNA System is a sensitive and easy-to-use fluorescence-based method for quantifying double-stranded DNA (dsDNA) in solution. The system is based on a proprietary fluorescent dye that binds specifically to dsDNA. When the dye binds to dsDNA, it emits fluorescence that can be measured using a fluorescence spectrophotometer or fluorometer. The amount of fluorescence is proportional to the amount of dsDNA in the solution.
Product Components and Storage Conditions
PRODUCT QuantiFluor® dsDNA System
SIZE 1mL
CATALOG NUMBER E2670
This system contains sufficient reagents for 2,000 assays at a 200µl scale. Includes:
- 25mL 20X TE Buffer (pH 7.5)
- 1mL QuantiFluor® dsDNA Dye
- 100 µg Lambda DNA Standard, 100 µg /mL
Storage Conditions: Product may arrive frozen. Upon receipt, store the QuantiFluor® dsDNA Dye at 2-10°C, protected from light. Store the Lambda DNA Standard, 100ug/ml, at 2-10°C. Do not refreeze the Lambda DNA Standard, 100
µg
/mL. Store the 20X TE Buffer at —30°C to +30°C.
How To Use
Using the QuantiFluor® dsDNA System typically involves several key steps to accurately quantify double-stranded DNA. Here is a general guide on how to use the system:
- Preparation: Make sure the system components are stored according to the recommended storage conditions. Thaw frozen components if necessary and ensure they are at the appropriate temperature for use.
- Sample Preparation: Prepare the DNA samples to be analyzed according to the specific guidelines provided with the system. Ensure that the samples are appropriately diluted to fall within the dynamic range of the assay.
- Dye Preparation: If required, prepare the QuantiFluor® dsDNA Dye according to the instructions provided. Be mindful of any specific reconstitution steps mentioned in the manual.
- Standards Preparation: Dilute the Lambda DNA Standard, if necessary, to create a standard curve for accurate quantification.
- Assay Protocol: Follow the step-by-step assay protocol outlined in the product manual. Typically, this involves combining the prepared samples, standards, and reagents in the provided assay plate or tubes according to the recommended volumes.
- Fluorescence Measurement: Use a fluorometer or a plate reader capable of measuring fluorescence to quantify the dsDNA in the samples. Follow the recommended measurement settings and parameters for accurate readings.
- Data Analysis: Utilize the provided software or follow the guidelines in the manual to analyze the obtained fluorescence data and calculate the concentration of the dsDNA samples based on the standard curve.
- Results Interpretation: Interpret the results based on the calculated DNA concentrations and use them for downstream applications as needed.
Always refer to the specific instructions provided in the QuantiFluor® dsDNA System manual for precise guidance on its usage and application. Additionally, if you encounter any challenges or require further assistance, consider reaching out to the technical support team provided by the manufacturer for expert guidance and troubleshooting.
The QuantiFluor® dsDNA System is an indispensable tool widely utilized in diverse molecular biology applications for the precise quantification of double-stranded DNA (dsDNA). With its high sensitivity and reliable performance, this system enables accurate measurements of DNA concentrations, ensuring the integrity and success of various experiments and analyses. Its versatility spans across essential processes, including PCR, DNA sequencing, cloning, electrophoresis, DNA binding studies, and quality control in next-generation sequencing (NGS) applications. Researchers rely on the QuantiFluor® dsDNA System for its robust capabilities, facilitating critical advancements in molecular biology research and enhancing the efficiency and accuracy of DNA analysis.
Techniques
The QuantiFluor® dsDNA System is commonly used for the quantification of double-stranded DNA (dsDNA) in various molecular biology applications. It employs fluorescence-based techniques to accurately measure DNA concentrations. The system typically utilizes the following techniques:
- Fluorescence Quantification: The QuantiFluor® dsDNA System uses fluorescent dyes that exhibit enhanced fluorescence when bound to dsDNA. By measuring the intensity of fluorescence emitted by the dye-DNA complex, the system can determine the concentration of dsDNA in a sample.
- Standard Curve Analysis: The system often involves the creation of a standard curve using known concentrations of a DNA standard, such as the provided Lambda DNA Standard. This standard curve helps establish a relationship between fluorescence intensity and DNA concentration, enabling the accurate quantification of unknown DNA samples.
- Fluorometry: The fluorescence of the dye-DNA complex is typically measured using a fluorometer or a plate reader equipped with fluorescence detection capabilities. The instrument detects and quantifies the emitted fluorescence, allowing for precise measurement of DNA concentrations in the samples.
By leveraging these fluorescence-based techniques, the QuantiFluor® dsDNA System provides researchers with a reliable and sensitive method for quantifying dsDNA, facilitating various molecular biology and biotechnology applications where accurate DNA concentration determination is critical.
Benefits of the QuantiFluor®dsDNA System
- Sensitive: The QuantiFluor® dsDNA System can detect as little as 25 pg of dsDNA per mL of solution.
- Easy to use: The QuantiFluor® dsDNA System is easy to use and requires no special equipment.
- Accurate: The QuantiFluor® dsDNA System is accurate and reproducible.
- Linear: The QuantiFluor® dsDNA System is linear over a wide range of DNA concentrations.
- Versatility: The QuantiFluor® dsDNA System can be used to quantify dsDNA in a variety of samples, including genomic DNA, PCR products, plasmid DNA, and viral DNA.
The QuantiFluor® dsDNA System is a rapid and accurate method for quantifying dsDNA. The system is also linear over a wide range of DNA concentrations, making it suitable for quantifying both small and large amounts of DNA.
Overview of QuantiFluor® dsDNA System
The QuantiFluor® dsDNA System is commonly used in various molecular biology applications where precise quantification of double-stranded DNA (dsDNA) is essential. Here are some typical uses of this product:
- PCR (Polymerase Chain Reaction): The QuantiFluor® dsDNA system is used to accurately determine the concentration of dsDNA in samples used in PCR reactions.
- DNA Sequencing: It is used to quantify DNA before preparation and sequencing steps, ensuring reliable and reproducible results.
- Cloning: This system enables the precise measurement of DNA concentrations when using cloning techniques.
- Electrophoresis: It is useful for quantifying DNA before and after electrophoresis, allowing the verification of the efficiency of DNA extraction or purification procedures.
- DNA Binding Studies: It can be used to quantify DNA in DNA binding studies, ensuring precise concentrations for further analysis.
- Molecular Biology Research: It finds applications in various molecular biology research studies where accurate quantification of DNA is crucial for reliable analyses.
These uses demonstrate the versatility of the QuantiFluor® dsDNA system in research laboratories and scientific environments, where accurate DNA measurement is essential for the success of experiments and analyses.
The QuantiFluor® dsDNA System is commonly used in various molecular biology applications where precise quantification of double-stranded DNA (dsDNA) is essential. Here are some typical uses of this product:
- DNA Sequencing: It is used to quantify DNA before preparation and sequencing steps, ensuring reliable and reproducible results.
- Cloning: This system enables the precise measurement of DNA concentrations when using cloning techniques.
- Electrophoresis: It is useful for quantifying DNA before and after electrophoresis, allowing the verification of the efficiency of DNA extraction or purification procedures.
- DNA Binding Studies: It can be used to quantify DNA in DNA binding studies, ensuring precise concentrations for further analysis.
- Molecular Biology Research: It finds applications in various molecular biology research studies where accurate quantification of DNA is crucial for reliable analyses.
These uses demonstrate the versatility of the QuantiFluor® dsDNA system in research laboratories and scientific environments, where accurate DNA measurement is essential for the success of experiments and analyses.
Library Preparation
Before sequencing, precise quantification of DNA is essential to prepare high-quality DNA libraries, ensuring reliable and reproducible results throughout the sequencing process.
Post-Sequencing Quantification
After sequencing, it can be used to accurately quantify the sequencing products and assess the overall sequencing yield.
Quality Control
The QuantiFluor® dsDNA system can be used to verify the quality of DNA before sequencing, ensuring high-quality DNA samples for accurate results during NGS analysis.
Amplification
Amplification is a crucial step in the NGS workflow, as it allows for the production of multiple copies of the DNA fragments, making them more amenable to the sequencing process and enabling the generation of robust and reliable sequence data.
Applications of the QuantiFluor® dsDNA System
The QuantiFluor® dsDNA System is a versatile tool that can be used for a variety of applications in molecular biology. The system is commonly used for:
- Quantifying dsDNA in genomic DNA preparations: The QuantiFluor® dsDNA System can be used to quantify dsDNA in genomic DNA preparations prior to restriction enzyme digestion, ligation, or PCR amplification.
- Quantifying dsDNA in PCR products: The QuantiFluor® dsDNA System can be used to quantify dsDNA in PCR products to determine the yield of the PCR reaction.
- Quantifying dsDNA in plasmid DNA preparations: The QuantiFluor® dsDNA System can be used to quantify dsDNA in plasmid DNA preparations prior to transfection or sequencing.
- Quantifying dsDNA in viral DNA preparations: The QuantiFluor® dsDNA System can be used to quantify dsDNA in viral DNA preparations prior to real-time PCR or other molecular analyses.
Overall, the QuantiFluor® dsDNA System is a valuable tool for researchers in molecular biology. The system is sensitive, easy to use, accurate, and versatile, making it an ideal choice for a wide range of applications.
Order Your QuantiFluor® dsDNA System Today!
The QuantiFluor® dsDNA System is a valuable tool for researchers in molecular biology. The system is sensitive, easy to use, accurate, and versatile, making it an ideal choice for a wide range of applications.
To learn more about the QuantiFluor® dsDNA System, please visit our website or contact us.
PCR (Polymerase Chain Reaction) and the QuantiFluor® dsDNA System
PCR (Polymerase Chain Reaction) is a widely used technique in molecular biology for amplifying a specific segment of DNA. The QuantiFluor® dsDNA System plays a crucial role in this process by accurately determining the concentration of double-stranded DNA (dsDNA) in the samples used during PCR reactions. This precise quantification is essential for ensuring the optimal amount of template DNA is available for the PCR process, leading to reliable and reproducible amplification results. By providing accurate measurements of dsDNA concentration, the QuantiFluor® dsDNA System contributes to the success and efficiency of PCR experiments, enabling researchers to achieve robust and consistent amplification of the target DNA region. This reliable quantification method aids in the precise control of the DNA input, contributing to the overall success of PCR-based applications in various research and diagnostic settings.
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